2.15 - Knife Sterilization without Fire Hazard

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In this video I teach you how to sterilize your knife with an only chemical ethanol sterilization. The benefit is, that you reduce the fire hazard and the blade does not get blunt. One need to sterilize the knife before performing agar mycelium work with your petri dishes when you want to clone your mycelium. There are two tubes involved. One is closeable for storage and the second one is open for immediate usage during the process. Here is how I apply the process: 1. The knife is in the closed storage tube, placed in ethanol. 2. When I need it, I put the knife holder into the still air box. 3. There I take it with the magnet out of the storage tube and place it into the shorter working tube. The ethanol mainly stays in the storage tube and only little bit drips on the ground of the working tube. The knife is clean, sterile and good to use. 4. When I cut several agar plates I sometimes have to use both hands. With the working tube I have a defined place for my knife. Whenever I do not need it, I put it there. (I do not put it back in to the ethanol filled storage tube.) Having a defined working place for the knife standardized the process and leads to lower contamination. 5. After finishing the cloning, I clean the working area and the knife. The clean knife is put back into the storage tube with ethanol. The tube is closed. <iframe width="560" height="315" src="https://www.youtube.com/embed/svAooK7qk8I" title="YouTube video player" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" allowfullscreen></iframe>