CRISPR / CAS9 Restriction enzyme set

prusaprinters

<p>Gene editing is an exciting new field of genetics. This is done with the CRISPER (Clustered regularly interspaced short palindromic repeats) and Cas sequence restriction enzymes.</p> <p>This is a remix of my DNA Restriction Enzymes set. (<a href="https://www.thingiverse.com/thing:1304176">http://www.thingiverse.com/thing:1304176</a>)<br/> For the sake of space I removed the space from the Phosphate groups, and condensed the CRISPER target sequence.</p> <p>The CRISPER 2 file has a large cutout in it. This will help you see the DNA sequence it is replacing, and it cuts the material and print time almost in half.<br/> You can use both pieces to show how CRISPER / Cas9 is used in gene editing.</p> <p>You can also expand this to talk about bioethics, cancer treatment and the human genome.</p> <h3>Print instructions</h3><p>Unassociated tags: #biologyProject, #Cas9, #CRISPER, #DNA, #enzyme, #geneEditing, #geneticPRoject, #MakerEdChallenge2, #RNA, #scienceProject</p> <h3>Category: Biology Print Settings</h3> <p><strong>Printer Brand:</strong> LulzBot</p> <p><strong>Printer:</strong> <a href="http://amzn.to/2nxubRO">TAZ 5</a></p> <p><strong>Rafts:</strong> Doesn't Matter</p> <p><strong>Supports:</strong> Doesn't Matter</p> <p><strong>Resolution:</strong> .2 - .3</p> <p><strong>Infill:</strong> 5%</p> <p><strong>Notes:</strong></p> <p>Break all sharp edges. Print 2 to show how CIRSPER is a palindrome sequence.</p> <p>Print the Cas9 the same as the RNA pieces from my other manipulative set. Print the CRIPSER in whatever color you have in bulk.</p> <p>To print this so the letters stand out, print the first base layers in a dark color (about 1.5 mm high), and then switch to a lighter filament about half way through. This will produce highly visible lettering that may help visually impaired students. Like the other restriction enzymes, you can also use a permanent marker to highlight the lettering.</p> <h3> Post-Printing</h3> <p><strong>Post Print</strong></p> <p>Print with my DNA / RNA Manipulative set.<br/> See the Restriction enzyme set for additional instructions.</p> <h3> How I Designed This</h3> <p><strong>Designed</strong></p> <p>This was a remix from my DNA / RNA manipulative sets. This set is the target sequence for CRISPER.</p> <p>More info on the sequence can be found here <a href="https://en.wikipedia.org/wiki/CRISPR">https://en.wikipedia.org/wiki/CRISPR</a></p> <p>To fit this onto a students desk, I compressed the target sequence by 1/2 to fit within the build volume of most 3D printers.</p> <p>Designed and remixed in tinkerCAD.</p> <h3> Custom Section</h3> <p><strong>PROJECT CRISPER</strong></p> <p>This is a project to expand upon others I have designed for genetics, DNA and RNA. You can cover DNA, RNA and gene editing with this set.</p> <p>Objectives<br/> Students will learn how CRISPER and Cas sequence RNA is used to edit genes.<br/> Students will examine how to change DNA with CRISPER.<br/> Students will examine the bioethics of using such technology.<br/> Students will examine the CRISPER method compared to other restriction enzymes.<br/> Students will explore how restriction enzymes work.</p> <p>Audiences<br/> This is for AP Biology, College biology, genetics, or medical professionals such as genetic councilors.</p> <p>Subjects<br/> AP Biology, College Biology, Genetics, Bioethics, Medical school</p> <p>Standards<br/> AP biology, College biology standards can be met with this set.</p> <p>Lesson<br/> See DNA / RNA manipulative, Restriction enzymes set for previous lessons.</p> <p>After learning about DNA, RNA, and restriction enzymes you can do the following.</p> <ol> <li>Model the CRISPER / CAS sequence and how it is used to edit genes. </li> <li>Explore how it is used to edit genes.</li> <li>Explore the bioethics of editing human and animal genes. </li> <li>Explore the positive and negative impacts of gene editing.</li> <li>Model and demonstrate how CRISPER can be used to produce a new gene sequence. </li> <li><p>Demonstrate how to treat a specific disorder with CRISPER.<br/> Duration:<br/> 1 - 3 weeks depending on the scope of your class.</p> <p>Preparation<br/> Students will need a set of the CRISPER / Cas9 pieces, as well as a full DNA / RNA manipulative set. You may want the Restriction enzymes set as well. You can 3D print the CRISPER in any filament you have in bulk. As Cas9 is a RNA sequence I recommend printing it the same color as the RNA pieces.</p> <p>You will need recent articles on CRISPER / Cas9 and articles on the bioethics of gene editing.</p> <p>References<br/> I started with <a href="https://en.wikipedia.org/wiki/CRISPR">https://en.wikipedia.org/wiki/CRISPR</a> for the target sequence. Other Cas sequences may come latter.</p> <p>Assessment<br/> Students should demonstrate with these pieces how to edit genes.<br/> Students should show the bioethics of this new technology.<br/> Students should show how gene editing happens.</p> <p>Future lesson plans and activities will be posted on my teachersPayTeachers website.</p> </li> </ol>