DNA Restriction Enzymes Expansion Set #MakerEdChallenge

prusaprinters

<p>Lesson Plans and lab work is now available on my teacher pay teacher site, <a href="https://www.teacherspayteachers.com/Product/3D-Printable-DNA-RNA-Manipulative-Lab-1-3452084">https://www.teacherspayteachers.com/Product/3D-Printable-DNA-RNA-Manipulative-Lab-1-3452084</a></p> <p>This is a set of restriction enzymes to expand upon my DNA/RNA Manipulative set. <a href="https://www.thingiverse.com/thing:1259352">http://www.thingiverse.com/thing:1259352</a></p> <p>These 4 restriction enzyme blocks can be used to model how they work, and how gene splicing / recumbent DNA works. The blocks also show how many restriction enzymes are palindromes with sticky ends.</p> <p>Your students will enjoy simulating genetic engineering with this expansion set.</p> <p>This features EcoRI, BamHI, Hindll, and Kpnl Restriction enzymes. There are over 600 of these restriction enzymes used in gene splicing.</p> <p>If you want to make your own restriction enzymes for gene editing, I punished an expansion set here <a href="https://www.thingiverse.com/thing:1321230">http://www.thingiverse.com/thing:1321230</a></p> <p>A partial list of restriction enzymes can be found here, <a href="https://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites">https://en.wikipedia.org/wiki/List\_of\_restriction\_enzyme\_cutting\_sites</a></p> <p>If you want other restriction enzymes made, I can make them on commission. PM me or contact me via stan@3dpt.club for details.</p> <h3> Print Settings</h3> <p><strong>Printer Brand:</strong></p> <p>LulzBot</p> <p><strong>Printer:</strong></p> <p>TAZ 5</p> <p><strong>Rafts:</strong></p> <p>Doesn't Matter</p> <p><strong>Supports:</strong></p> <p>Doesn't Matter</p> <p><strong>Resolution:</strong></p> <p>.3-.4 mm</p> <p><strong>Infill:</strong></p> <p>5%</p> <p><strong>Notes:</strong></p> <p>I printed them in clear filament so they can lay over a DNA sequence and you can find where they match.</p> <p>Break all sharp edges. If you can't see the lettering, use a permanent marker to highlight the edge of the letters.</p> <p>Print 2 of each enzyme so you can model the cutting pattern and cleaved fragments.</p> <h3> Post-Printing</h3> <p><strong>Clean up</strong></p> <p>After printing break any sharp edges. I found a emery board works great for rough edges. If you print clear, use a permanent marker to highlight the edge of the lettering.</p> <h3> How I Designed This</h3> <p><strong>Design and printing</strong></p> <p>These were designed in TinkerCAD based on the previous DNA / RNA file.</p> <p>They were designed to fit as one piece so you can see how they work, and save time building them with the other set.</p> <p>If you want to expand and make your own, DL my expansion set here <a href="https://www.thingiverse.com/thing:1321230">http://www.thingiverse.com/thing:1321230</a></p> <h3> Custom Section</h3> <p><strong>Project: Gene Splicing and Restriction Enzymes</strong></p> <p>This Is to expand upon the DNA / RNA manipulative set. Your students will see how gene splicing, and how restriction enzymes are used to splice new genes into an organism.</p> <p>You can visit <a href="https://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites">https://en.wikipedia.org/wiki/List\_of\_restriction\_enzyme\_cutting\_sites</a> for a list of restriction enzymes and their cutting sites. You can make your own with this expansion set <a href="https://www.thingiverse.com/thing:1321230">http://www.thingiverse.com/thing:1321230</a></p> <p><strong>Objectives</strong></p> <p>Students will understand what genetic modification is.<br/> Students will model how restriction enzymes work.<br/> Students will model gene splicing.<br/> Students will demonstrate how to alter DNA.<br/> Students will discuss the ethics and implications of genetic modification.<br/> Students will discuss genetically modified organisms (GMO's) and agriculture.<br/> Students will show their knowledge of DNA and RNA.<br/> Students will model how mecumbant DNA works.<br/> Students will show sticky ends, fragments, and cleavage of DNA.</p> <p><strong>Audiences</strong></p> <p>This is for AP Biology, College Bio, Medical students, and home school students.<br/> This can also be used by genetic councilors, doctors, and medical students.</p> <p><strong>Preparation</strong></p> <p>Students should learn about DNA and RNA.<br/> Students should understand vectors and the concepts of gene splicing.<br/> Students should know about mutations.<br/> Students should know what paladrones are, and how they appear in DNA.<br/> Students should know what an enzyme does.<br/> Students should know the base pairs, what direction (5- 3) DNA is read.</p> <p>To use these restriction enzymes, you should print up 2 of them along with the DNA / RNA set.</p> <p>A activity package will soon be available, and sold on my Teacher Pay Teacher page. Printed sets of restriction enzymes will be for sale on my etsy page.</p> <p><strong>Steps!</strong></p> <p>a. Build a model of DNA matching the restriction enzyme.<br/> b. Model how the restriction enzyme cuts the DNA along specific base pairs.<br/> c. Show where the sticky ends are.<br/> d. Use the enzymes to insert a new DNA sequence.<br/> e. Discuss gene splicing. Show organisms that have undergone this procedure.<br/> f. Discuss the ethics on gene splicing.</p> <p><strong>Results</strong></p> <p>To demonstrate knowledge of this, have the students work in small groups to demonstrate the process or teach it to others.<br/> You can have the students make a stop-motion video of the process of gene splicing.<br/> You can also have them show what they have learned by modeling DNA replication, RNA transcription, protein synthesis, and gene splicing to produce new proteins.<br/> You can tie it to pop science by having them watch sections of the film Jurassic Park / Jurassic World that discuss what they did to create the dinosaurs. With that have the students model what the scientist in the film did.</p> Category: Biology