T7 DNA replisome

prusaprinters

<p>Surface model of the T7 replisome complex as described in <a href="https://science.sciencemag.org/content/sci/early/2019/01/23/science.aav7003.full.pdf">Gao et al., Science 10.1126/science.aav7003</a> (2019). All components were printed separately and glued. Proteins subunits were printed in PLA, while nucleic (DNA, RNA, and dTTP) in white TPU. The helicases complex from PDB ID <a href="https://www.rcsb.org/structure/6N7V">6N7V</a> is printed in gray, leading strand polymerase from PDB ID <a href="https://www.rcsb.org/structure/6N7W">6N7w</a> is printed in green and cooper, lagging strand pol from PDB ID <a href="https://www.rcsb.org/structure/6N9V">6N9V </a>is printed in red, blue, and yellow. All nucleic acids and relevant ligands were printed in white. RNA was painted black and dTTP was painted blue. An artificial DNA double-helix was generated in Chimera, the two strands were printed separately and used to connect and assemble the subunits (including the lagging strand loop). The same artificial DNA double-helix was printed 2x more times and used to enlarge the template DNA, leading and lagging strands.</p><p>All printed files are provided as stl. A .py session file usable in Chimera is also provided to help the assembly. The model was created to help me teaching Biochemistry and Molecular Biology at the undergrad level.</p><p>Segmentation Software : Prusa Slicer</p><p>3D Modeling/CAD Software : Chimera</p><p>Model Origin : Hybrid: data + illustration</p><p>Printer Technology/Material : Polylactic Acid (PLA)/ Flexible</p><p>Printer Make/Model : Creality Ender 5 Pro</p><p>Print Units : mm</p><p>Scale At Given Print Units : 100x</p><p>Pre- and Postprocessing Instructions&nbsp;</p><p>Printed at 0.2 resolution with 2 wall lines and 5% infill. The printer was modified with a direct drive extruder in order to print TPU. Extensive supports are required but were rather easy to remove.</p>