Human proteasome 20S core particle

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This is the structure of the 20S core particle of the human 26S proteasome, based on the structure published by Huang et al. (Nature Structural & Molecular Biology 23, 778–785 (2016) doi:10.1038/nsmb.3273). The PDB file representing the structure determined by the researchers was edited to remove the 19S regulatory subunits and the surface rendered using Chimera (http://www.cgl.ucsf.edu/chimera/), and exported as an .STL file. Print Settings Printer: ORD Solutions RoVa3D Rafts: Yes Supports: Yes Resolution: .2 mm Infill: 40% Notes: Printed using ABS plastic Post-Printing Acetone vapour polished after removing raft and supports. How I Designed This Based on the PDB file 5GJR available from the RSCB protein data bank (http://www.rcsb.org/) Overview and Background Transverse and cross sections through the proteasome core particle were used in a Cell Biology class to illustrate the mechanism of protein recycling in cells. Lesson Plan and Activity This model was used in conjunction with first a lecture-based introduction to protein quality control (protein folding, HSPs, ubiquitnylation/degrons, and proteasome regulation), followed by detailed analysis of several recent research papers on the regulation of protein recycling and its relationship to diseases like Parkinson's. References An atomic structure of the human 26S proteasome Xiuliang Huang, Bai Luan, Jianping Wu & Yigong Shi Nature Structural & Molecular Biology 23, 778–785 (2016) doi:10.1038/nsmb.3273