Mason Jar Bioreactor

thingiverse

Mason Jar Bioreactor Make a low-cost bioreactor with common materials! For the use with wide mouth Mason jars. This is still a work in progress Bioreactors are typically used when doing tissue engineering to grow the cells needed for seeding after decellularization of some type of organic matter, typically fruit, organs, or muscles. I designed this bioreactor to be very low cost and to be printed on a resin printer as to an ideal environment inside the reactor. It can be printed on a common FDM machine as well but I would keep it in a flow hood. There is a place for a bearing in the middle of the lid which takes a 16mm bearing. This is for a stirrer if you wanted to use one but it's not really needed. You can print a cap for the middle if you will not be using it. The procedure can be found here: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0097835#s2 I used a strawberry in my decellularization but it can be used with a lot of other things. Some other great reads: http://www.pellinglab.net/using-fruits-for-in-vitro-3d-cell-culture/ http://ossificatorium.blogspot.com/2013/10/biomaterial-processes-diy-tissue.html