Replication- Gene Synthesizer by Kong, Jacobson
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Replication of a four chamber microfluidic chip for synthesizing DNA. Chambers have a water cooling jacket and a control layer to cool the reaction volume. Fabrication by PDMS casting over stereo lithography etched master molds (flow layer and control layer)Overall dimensions: 40 x 25 x 2 mm, 100 µm wide flow input channels, 300 µm wide control channels.Materials:PDMS, glassInspiration/reference: Kong, Jacobson et al., Parallel gene synthesis in a microfluidic device, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885655/pdf/gkm121.pdfMetaFluidics: Microfluidic Gene Synthesizer, https://metafluidics.org/devices/microfluidic-gene-synthesizer/
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